Anti-DCP1A Rabbit Polyclonal Antibody for WB, ELISA - Q9NPI6

Core Product Specifications and Parameters

Biological Background: DCP1A Function and Localization

• DCP1A, also known as SMIF or SMAD4IP1, is a 582-amino acid protein encoded by the DCP1A gene and belongs to the mRNA decapping enzyme family.
• It is necessary for normal mRNA turnover and nonsense-mediated mRNA decay, catalyzing the removal of the 7-methylguanosine cap from mRNA to generate 5'-phosphorylated mRNA and 7m-GDP. Related references: PMID:12417715
• DCP1A acts as a transcriptional co-activator (SMIF) that enhances TGFB1-induced transactivation of target genes through interaction with SMAD4. Related references: PMID:11836524
• Essential for embryonic development, with genetic studies highlighting its critical role in early embryogenesis. Related references: PMID:33813271
• Subcellularly, DCP1A is predominantly cytoplasmic and localizes to processing bodies (P-bodies), but it is also detected in the nucleus.
• It is broadly expressed across tissues, including heart, brain, placenta, lung, skeletal muscle, liver, kidney, and pancreas.
• Post-translational modifications include phosphorylation and methylation, and the gene undergoes alternative splicing, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 303–582), which may aid in detecting full-length DCP1A and certain isoforms. For Western blot, consider using whole cell lysates from human, mouse, or rat tissues known to express DCP1A.
• When performing ELISA, titrate the antigen coating concentration and consider pairing with a suitable capture antibody for sandwich assays. Validate specificity by knockdown or overexpression in relevant cell lines.
• Due to its role in TGF-beta signaling, treat cells with TGFB1 to induce DCP1A transcriptional activity before sampling if studying its co-activator function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material supplier, providing diagnostic manufacturers and researchers with validated antibodies, including this polyclonal antibody targeting DCP1A for investigations into mRNA metabolism and TGF-beta signaling. With expertise in antibody development, bulk reagent supply, and sourcing of rare targets, CamelBio supports every step from concept to clinical application.