Anti-DCP1A Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NPI6

Core Product Specifications and Parameters

Biological Background: DCP1A Function and Localization

• DCP1A (mRNA-decapping enzyme 1A) is a hydrolase that removes the 7-methyl guanine cap from mRNA molecules, generating 5'-phosphorylated mRNA and 7m-GDP, necessary for normal mRNA turnover and nonsense-mediated mRNA decay. Related references: PMID:12417715
• It also functions as a transcriptional co-activator (named SMIF) that contributes to transactivation of target genes after TGFB1 stimulation. Related references: PMID:11836524
• DCP1A is essential for embryonic development. Related references: PMID:33813271
• Localizes to the cytoplasm, specifically in P-bodies, and also in the nucleus.
• Detected in multiple tissues including heart, brain, placenta, lung, skeletal muscle, liver, kidney, and pancreas.
• The protein undergoes post-translational modifications such as phosphorylation and methylation; it is a member of the reference proteome.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 483–582 (C-terminal region), so the antibody is expected to recognize full-length DCP1A. Confirm specificity by assessing band size and, if needed, knock-down/knock-out validation.
• For Western blot (WB), a band around 63 kDa is expected. Use positive controls from human or mouse cell lines known to express DCP1A.
• For immunohistochemistry (IHC-P), validate the antibody in paraffin-embedded tissue sections with known DCP1A expression, such as heart or placenta.
• For immunofluorescence (IF/ICC), DCP1A typically shows cytoplasmic P-body puncta and may also show nuclear staining; optimize fixation and permeabilization conditions for the cell type used.
• For ELISA, the synthetic peptide immunogen can be used as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined, one-stop sourcing platform for IVD raw materials. For DCP1A and associated mRNA decapping or TGF‑β signaling research, we can supply validated antibody pairs, optimized monoclonal antibodies like this anti-DCP1A clone, bulk ancillary reagents, and bespoke sourcing for rare targets. Partner with CamelBio to efficiently bridge your concept-to-clinic development needs.