Anti-DCN (Decorin) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07585

Core Product Specifications and Parameters

Biological Background: Decorin Function and Localization

• Decorin (DCN), also known as Bone proteoglycan II or PG40, is a small leucine-rich proteoglycan (SLRP) that plays a critical role in extracellular matrix organization.
• It may regulate collagen fibrillogenesis by affecting the rate of fibril formation, thereby influencing tissue architecture and mechanical properties.
• Alternative splicing of the DCN gene produces multiple isoforms, and mutations in this gene have been linked to corneal dystrophy.
• Decorin is a secreted protein found in the extracellular matrix and body fluids; it has been detected in placenta, cerebrospinal fluid, fibroblasts, and urine at the protein level.
• As a proteoglycan, decorin undergoes extensive post-translational modifications, including glycosylation and addition of glycosaminoglycan chains, contributing to its diverse biological functions.
• The protein contains leucine-rich repeats and disulfide bonds, which are essential for its structural integrity and interactions with other matrix components.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 31-359 of human decorin (NP_001911.1); for WB, a band near 40 kDa is expected, but glycan modifications may cause a diffuse migration pattern.
• For IHC and IF applications, consider optimizing antigen retrieval and blocking conditions due to decorin's association with glycosaminoglycans in the extracellular matrix.
• Validate antibody specificity in your sample system using positive controls expressing decorin, such as human fibroblasts or placenta tissue.
• Cross-reactivity with mouse and rat has been reported; verify expected band size in these species as molecular weight may slightly vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with a streamlined supply chain for IVD raw materials, including validated polyclonal and monoclonal antibodies for extracellular matrix targets like decorin. Our one-stop platform also offers bulk ancillary reagents and custom sourcing services to accelerate your assay development from research to clinical application.