Anti-DCLRE1C Rabbit Polyclonal Antibody for WB, ELISA - Q96SD1

Core Product Specifications and Parameters

Biological Background: Protein Artemis (DCLRE1C) Function and Localization

• DCLRE1C encodes protein Artemis (also known as SNM1C, A-SCID), a multifunctional nuclease essential for DNA repair and immune diversity.
• Artemis is a critical component of the non-homologous end joining (NHEJ) pathway, required for repairing DNA double-strand breaks (DSBs) and for V(D)J recombination, the process that assembles immunoglobulin and T-cell receptor genes. Related references: PMID:11336668, PMID:11955432, PMID:12055248
• In isolation, Artemis exhibits 5'-3' exonuclease activity on single-stranded DNA; upon phosphorylation and complex formation with DNA-PKcs (PRKDC), it acquires endonucleolytic activity on 5' and 3' hairpins and overhangs, which is crucial for opening hairpin-sealed coding ends during V(D)J recombination. Related references: PMID:11955432, PMID:15071507, PMID:15574326
• Artemis is also required for the repair of complex DSBs induced by ionizing radiation, which demand extensive end-processing before ligation by NHEJ. Related references: PMID:15456891, PMID:15468306, PMID:15574327
• Artemis localizes predominantly to the nucleus, consistent with its role in DNA repair and recombination.
• Widely expressed at the mRNA level, with highest levels in kidney, lung, pancreas, and placenta; notably, expression is not elevated in primary lymphoid tissues such as thymus or bone marrow despite its role in V(D)J recombination.
• Mutations in DCLRE1C are linked to severe combined immunodeficiency (SCID) with sensitivity to ionizing radiation (RS-SCID), and the protein is classified under DNA damage, DNA repair, nuclease, and phosphoprotein keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa1–300) of human DCLRE1C, which shares high sequence homology across human, mouse, and rat, supporting its predicted cross-reactivity. For Western blot, validate band specificity using positive controls such as HeLa or HEK293 cell lysates, expecting a band at ~78 kDa.
• For ELISA, consider pairing this polyclonal antibody with a DCLRE1C-specific monoclonal antibody for sandwich assays; optimize coating and detection conditions using recombinant protein standards.
• Since Artemis forms complexes with PRKDC, be aware that native protein may migrate differently under non-reducing conditions; always run appropriate molecular weight markers and include negative controls.

CamelBio: Your One-Stop Sourcing Bridge

For your DCLRE1C-targeted assay development, CamelBio serves as your one-stop sourcing partner, supplying polyclonal antibodies like this DCLRE1C pAb, along with matched antibody pairs, recombinant antigens, and bulk ancillary reagents. Our integrated raw-material solutions help streamline your DNA repair and immunology research projects, from concept to clinical application. Contact CamelBio to access rare target materials and technical support for your IVD development needs.