Anti-DCK Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27707

Core Product Specifications and Parameters

Biological Background: DCK Function and Localization

• DCK encodes deoxycytidine kinase, which also phosphorylates deoxyadenosine and deoxyguanosine, playing a central role in the nucleoside salvage pathway.
• The enzyme displays broad substrate specificity without strict chiral discrimination.
• It is essential for the activation of numerous nucleoside analog prodrugs widely employed as antiviral and chemotherapeutic agents. Related references: PMID:12808445 PMID:18377927 PMID:19159229
• Subcellular localization is predominantly nuclear, aligning with its function in DNA precursor metabolism.
• Key structural features include ATP-binding and kinase domains critical for catalytic phosphorylation; the protein is identified as a phosphoprotein by proteomics.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning full-length human DCK (aa 1–260), suggesting polyclonal recognition of multiple epitopes, potentially detecting both phosphorylated and unphosphorylated forms. Consider appropriate negative controls.
• For WB, expect a band near 31 kDa. Enrich nuclear fractions to improve signal intensity; validate specificity using positive (e.g., proliferating cell lines) and negative controls.
• In IHC-P and IF/ICC, confirm nuclear staining in known positive tissues/cells. Optimize antigen retrieval and blocking conditions for your sample type.
• For ELISA, use as capture or detection antibody; include cross-reactivity controls given the antibody recognizes human, mouse, and rat DCK.
• Cross-reacts with mouse and rat DCK; preliminary validation in the species of interest is advised to confirm specificity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined supply channel for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from target discovery to assay production. The DCK target is pivotal in cancer and antiviral research; our platform offers access to this polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Let CamelBio fuel your next breakthrough with reliable, research-grade immunoreagents.