Anti-DCK Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P27707

Core Product Specifications and Parameters

Biological Background: DCK Function and Localization

• DCK, also known as deoxycytidine kinase, deoxyadenosine kinase, and deoxyguanosine kinase, is a 30.5 kDa nuclear enzyme that belongs to the kinase and transferase families.
• It phosphorylates deoxycytidine, deoxyguanosine, and deoxyadenosine, displaying broad substrate specificity independent of chirality.
  Related references:
  PMID:12808445, PMID:18377927, PMID:19159229
• DCK is a critical enzyme for the activation of numerous nucleoside analog prodrugs used in antiviral and anticancer therapies (e.g., gemcitabine, lamivudine).
  Related references:
  PMID:12808445
• It localizes predominantly to the nucleus, consistent with its role in nucleotide metabolism for DNA synthesis and repair.
• Post-translational modifications include phosphorylation (as indicated by proteomics data), which may regulate its activity.
• Keywords associated with DCK include ATP-binding, nucleotide-binding, kinase, transferase, nucleus, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen spans the full length of human DCK (1-260), so the antibody recognizes the native folded protein; consider using denaturing conditions for Western blot to ensure linear epitope exposure.
• For IHC-P, antigen retrieval may be required; validate with positive controls (e.g., proliferating tissues) as DCK is ubiquitously expressed but elevated in replicating cells.
• Due to high sequence homology (cross-reactivity with mouse and rat), this antibody is suitable for rodent model studies; however, verify band size differences if any (human DCK is 30.5 kDa, but predicts 31kDa on blot).
• ELISA: Dilution optimization is necessary; use recombinant DCK protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, robust monoclonal antibodies like this anti-DCK clone, and bulk ancillary reagents. We specialize in bridging the gap from discovery to diagnostic development, supporting research in nucleoside metabolism and cancer biomarkers. Whether you need custom antibody production or rare target sourcing, our one-stop platform accelerates your path from concept to clinic.