Anti-DCAMKL1/DCLK1 Polyclonal Antibody for WB, IF-P, ELISA - O15075

Core Product Specifications and Parameters

Biological Background: DCLK1 Function and Localization

• DCLK1 (Doublecortin-like kinase 1) is a serine/threonine-protein kinase containing doublecortin domains, implicated in calcium-signaling pathways controlling neuronal migration in the developing brain.
• It may also participate in functions of the mature nervous system.
• In fetal tissues, DCLK1 is highly expressed in the brain, and detectable in lung and liver; it is not expressed in fetal kidney.
• In adult tissues, it is ubiquitously expressed in the brain and detectable in heart, liver, spleen, thymus, prostate, testis, ovary, small intestine, and colon.
• Alternative splicing generates type A isoforms, predominantly expressed in fetal brain, and type B isoforms, present in both fetal and adult brain.
• Classified as a developmental protein, it is involved in neurogenesis and differentiation.
• The protein is a phosphoprotein and binds ATP, consistent with its kinase activity.

Experimental Guidance and Technical Tips

• For Western Blot (WB), expect a band around 82 kDa. Use lysates from neuronal tissues or DCLK1-expressing cell lines, and validate loading controls.
• For Immunofluorescence-Paraffin (IF-P), optimize fixation and antigen retrieval conditions for your sample type, particularly in brain or embryo tissues.
• Since this is a polyclonal antibody, perform lot-specific dilution optimization for each application (e.g., ELISA) to ensure consistent signal-to-noise ratios.
• Cross-reactivity with mouse and rat indicates suitability for models; however, verify band size and specificity in your experimental system.
• The immunogen corresponds to amino acids 590-740 near the C-terminus; epitope accessibility may vary depending on protein conformation and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-DCLK1 pAb, for neuroscience and developmental biology applications. With expertise in sourcing rare targets and bulk ancillary reagents, we support every stage from concept to clinic, ensuring reliable supply for your assay development needs.