Anti-DCAF7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61962

Core Product Specifications and Parameters

Biological Background: DCAF7 Function and Localization

• DCAF7, also known as WDR68 or AN11, is a conserved WD repeat-containing protein that acts as a substrate receptor in the CUL4-DDB1 E3 ubiquitin ligase complex, potentially targeting specific proteins for ubiquitination.
• It plays a critical role in craniofacial development, acting upstream of the EDN1 pathway to regulate formation of the upper jaw equivalent.
• DCAF7 associates with DIAPH1 and modulates GLI1 transcriptional activity, linking it to Hedgehog signaling and cellular differentiation.
• The protein is implicated in normal and disease skin development, although the exact mechanisms remain under investigation.
• Subcellularly, DCAF7 localizes to both the cytoplasm and the nucleus, consistent with its roles in signal transduction and nuclear transcription regulation.
• Alternative splicing produces multiple isoforms, suggesting tissue- or context-specific functions.
• Keywords from UniProt include developmental protein, Ubl conjugation pathway, and WD repeat, highlighting its involvement in protein degradation and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing the full-length human DCAF7 (amino acids 1-342). This design suggests the antibody may recognize multiple epitopes, offering robust detection.
• For Western blot, DCAF7 migrates at approximately 39kDa. Use appropriate positive controls such as HeLa or HEK293 cell lysates, and validate in both human and mouse samples due to confirmed cross-reactivity.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions; consider using 4% PFA followed by 0.1% Triton X-100. The protein's dual cytoplasmic and nuclear localization may be observed.
• For ELISA applications, employ the recombinant immunogen as a standard or use cell lysates; ensure the antibody concentration is titrated for optimal signal-to-noise ratio.
• Given the antibody's polyclonal nature, confirm specificity with appropriate controls, including knockdown or knockout lysates if available, and note that batch-to-batch consistency should be monitored.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to a broad portfolio of IVD raw materials, including this anti-DCAF7 polyclonal antibody for developmental biology and ubiquitination research. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare target raw materials. Trust CamelBio to support your projects from concept to clinic with reliable and comprehensive solutions.