Anti-DCAF7 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P61962

Core Product Specifications and Parameters

Biological Background: DCAF7 Function and Localization

• DCAF7 (DDB1- and CUL4-associated factor 7), also known as WDR68 or AN11 homolog, is a 342-amino acid protein with a predicted molecular weight of ~39 kDa. It contains WD repeats and is involved in craniofacial development by acting upstream of the EDN1 pathway, which is essential for upper jaw (palatoquadrate) formation.
• The protein associates with DIAPH1 and modulates GLI1 transcriptional activity, potentially influencing developmental signaling and skin development.
• DCAF7 may function as a substrate receptor for the CUL4-DDB1 E3 ubiquitin-protein ligase complex, implicating it in ubiquitin-dependent protein turnover.
• Subcellularly, DCAF7 localizes to both the cytoplasm and nucleus, consistent with its roles in signaling and gene regulation.
• The gene undergoes alternative splicing, and the protein is classified under developmental proteins and ubiquitin conjugation pathway components (UniProt keywords).

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of DCAF7 is ~39 kDa. Use appropriate loading controls and validate detection in relevant human, mouse, or rat tissue or cell lysates.
• For immunohistochemistry (IHC-P), consider antigen retrieval optimization and inclusion of isotype controls to confirm staining specificity.
• In ELISA applications, pairing this antibody with another DCAF7-specific antibody may enhance sensitivity; titrate both capture and detection reagents for optimal signal-to-noise ratio.
• Since the immunogen is a synthetic peptide within amino acids 200-300, the antibody is expected to recognize human DCAF7 and cross-react with mouse and rat homologs, but endogenous expression levels should be confirmed in the target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research institutions by providing a one-stop sourcing solution for raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target reagents. This anti-DCAF7 rabbit monoclonal antibody is suited for studies in craniofacial development and ubiquitin-proteasome pathways, complementing our extensive catalog designed to streamline assay development from concept to clinic.