Anti-DCAF7 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61962

Core Product Specifications and Parameters

Biological Background: DCAF7 Function and Localization

• DCAF7, also known as WDR68 or AN11 homolog, is a WD repeat-containing protein that functions as a substrate receptor for the CUL4-DDB1 E3 ubiquitin-protein ligase complex.
• It plays a critical role in craniofacial development, acting upstream of the EDN1 signaling pathway and required for upper jaw formation (palatoquadrate).
• DCAF7 associates with DIAPH1 and modulates GLI1 transcriptional activity, linking it to Hedgehog signaling.
• It may participate in normal and pathological skin development.
• Localizes to both cytoplasm and nucleus, consistent with its roles in signaling and ubiquitination.
• Belongs to a family of DDB1- and CUL4-associated factors (DCAFs) that mediate substrate specificity in ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full length of DCAF7 (residues 1-342), suggesting the antibody may detect multiple isoforms; validate in your model system.
• For western blot, expect a band at ~39 kDa; use lysates from tissues with known DCAF7 expression, such as craniofacial or skin samples.
• In immunohistochemistry (IHC-P) or immunofluorescence, confirm specificity with appropriate controls, noting the cytoplasmic and nuclear localization.
• ELISA application may require optimization of antibody pair and sample matrix; consider testing in biologically relevant fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials and technical services, supporting every stage from concept to clinic. This anti-DCAF7 polyclonal antibody, targeting a key regulator of craniofacial and ubiquitin pathways, reflects our commitment to supplying high-quality reagents for developmental biology and disease research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development.