Anti-DCAF13 Polyclonal Antibody for WB, ELISA - Q9NV06

Core Product Specifications and Parameters

Biological Background: DCAF13 Function and Localization

• Protein identity: DDB1- and CUL4-associated factor 13, also known as WD repeat and SOF domain-containing protein 1, encoded by the DCAF13 gene in humans.
• Participates in 18S rRNA processing and ribosome biogenesis as part of the small subunit (SSU) processome, critical for oocyte nucleolar transition. Related references: PMID:34516797, PMID:30283081
• Functions as substrate-recognition component of DCX E3 ubiquitin ligase, targeting SUV39H1/2 for degradation to remove H3K9me3 and facilitate zygotic gene expression, and targeting PTEN to support oocyte maturation. Related references: PMID:16949367, PMID:30111536, PMID:31492966
• Essential for embryo preimplantation development, meiotic progression, and endometrial decidualization. Related references: PMID:35932979
• Subcellular localization: Nucleus, nucleolus.
• Features WD repeats and is subject to acetylation and alternative splicing; involved in the ubiquitin conjugation pathway.
• Expressed in endometrium during decidualization; downregulated in preeclampsia decidual tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 298-597) of human DCAF13, which may provide specificity for detection of full-length and potentially cleaved products. Validate in relevant sample types.
• For Western blot, a band around 51 kDa is expected; consider using appropriate molecular weight markers and loading controls.
• For ELISA, the antibody can be used to detect DCAF13 in cell lysates or recombinant protein samples; optimize coating conditions.
• Cross-reactivity with mouse and rat allows testing in rodent models; confirm with appropriate species controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing platform for IVD raw materials. This DCAF13 polyclonal antibody is suited for research in ribosome biogenesis, ubiquitination, and reproductive biology. Beyond this product, CamelBio offers validated antibody pairs, bulk ancillary reagents, and rare target sourcing to support your full development pipeline.