Anti-DCAF12 Polyclonal Antibody for WB and ELISA - Q5T6F0

Core Product Specifications and Parameters

Biological Background: DCAF12 Function and Localization

• DCAF12 (DDB1- and CUL4-associated factor 12), also known as CT102, TCC52, WDR40A, is a WD repeat-containing protein.
• It functions as a substrate-recognition component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complex, specifically recognizing C-terminal degrons (C-degrons) to target proteins for ubiquitination and degradation. Related references: PMID:16949367, PMID:16964240, PMID:29779948
• The DCX(DCAF12) complex recognizes diglutamate (Glu-Glu) C-termini in substrates such as MAGEA3, MAGEA6, and CCT5, leading to their degradation; this activity is required for starvation-induced autophagy. Related references: PMID:29779948, PMID:31267705
• DCAF12 also recognizes an alternative glutamate-leucine (Glu-Leu) C-degron, as seen in MOV10, and controls MOV10 protein levels in spermatogenesis and activated T cells. Related references: PMID:34065512
• Subcellular localization includes cytoplasm, centrosome (microtubule organizing center), and nucleus.
• Tissue expression is restricted to normal testis, but it is highly expressed in lung cancer tissues and some cancer cell lines. Related references: PMID:18957058
• Keywords include 3D-structure, cytoplasm, cytoskeleton, nucleus, phosphoprotein, proteomics identification, reference proteome, repeat (WD repeat), and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 85 amino acids of DCAF12; consider this when designing epitope-specific experiments.
• For Western blot, the predicted molecular weight is ~51 kDa; however, post-translational modifications (e.g., phosphorylation) may affect migration.
• DCAF12 is involved in ubiquitination and autophagy pathways; consider relevant treatment conditions (e.g., starvation) and appropriate controls in functional assays.
• The antibody is suitable for ELISA; validate in the relevant sample system with appropriate antigen presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-DCAF12 polyclonal antibody supports research into ubiquitin ligase biology, autophagy, and cancer/testis targets. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your development pipelines.