Anti-DC-SIGNR/CD299 Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - Q9H2X3

Core Product Specifications and Parameters

Biological Background: DC-SIGNR/CD299 Function and Localization

• DC-SIGNR/CD299 (C-type lectin domain family 4 member M) is also known as L-SIGN, DC-SIGN2, and CD209L. It belongs to the C-type lectin receptor family and shares homology with DC-SIGN (CD209).
• Functions as a pathogen-recognition receptor involved in peripheral immune surveillance, particularly in the liver. It mediates endocytosis of pathogens for lysosomal degradation.
• Acts as an attachment receptor for numerous viruses, including HIV-1, hepatitis C virus, SARS-CoV, Ebola virus, influenza virus, and others, facilitating viral entry into host cells.
• Recognizes ICAM3 via mannose-like carbohydrate binding, contributing to dendritic cell-T cell interactions.
• Localizes to the cell membrane and can also be secreted. The secreted form is produced by alternative splicing.
• Predominantly expressed in liver sinusoidal endothelial cells and lymph node, with additional expression in placental endothelium, type II alveolar cells, and lung endothelial cells. Not found in macrophages.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are important for ligand binding and structural stability. Its molecular weight varies due to glycosylation, observed between 24 and 45 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 71-399), enabling detection of both membrane-bound and secreted DC-SIGNR isoforms. For Western blot, validate in liver endothelial cell lines as positive controls; the glycosylated protein migrates between 24-45 kDa.
• For IF/ICC, expect pronounced cell surface staining; permeabilization may reveal intracellular pools. For flow cytometry, use live cells for surface expression or fixed/permeabilized cells for total detection.
• In ELISA applications, the antibody can serve as capture or detection reagent; include recombinant protein standards for quantitation.
• Cross-reactivity is restricted to human; confirm suitability in your assay system when working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-DC-SIGNR/CD299 monoclonal antibody is part of our extensive portfolio for infectious disease research, supporting studies on pathogen recognition and viral entry mechanisms. For scaled-up needs, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions tailored to your development pipeline.