Anti-DBP Rabbit Polyclonal Antibody for WB and ELISA - Q10586

Core Product Specifications and Parameters

Biological Background: DBP Function and Localization

• DBP (D site-binding protein) is also known as Albumin D box-binding protein, Albumin D-element-binding protein, and Tax-responsive enhancer element-binding protein 302 (TaxREB302).
• It functions as a transcriptional activator that recognizes and binds to the DNA sequence 5'-RTTAYGTAAY-3' in promoters of genes such as albumin, CYP2A4, and CYP2A5.
• Although not essential for circadian rhythm generation, DBP modulates important clock output genes and may be a direct target for regulation by the circadian pacemaker component clock.
• DBP may affect circadian period and sleep regulation.
• Subcellular localization: predominantly nuclear.
• It is ubiquitously expressed in tissues, including the suprachiasmatic nuclei (SCN), with strong circadian rhythmicity.
• Keywords: Activator, Biological rhythms, DNA-binding, Nucleus, Phosphoprotein (indicating potential post-translational regulation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human DBP sequence (aa 1-325), which may provide broad epitope recognition.
• For Western blot, a band around 34 kDa is expected based on the theoretical molecular weight of 34349 Da; validate in the relevant sample system.
• As a polyclonal antibody, lot-to-lot consistency should be assessed; include appropriate controls and optimize dilutions for each application.
• ELISA can be used for quantitative detection; consider pairing with a suitable secondary antibody and validate assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, supporting your research on transcription factors and circadian biology with this high-quality anti-DBP polyclonal antibody. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing for rare targets to streamline your assay development from concept to clinic.