Anti-DBNL Polyclonal Antibody for WB, ELISA - Q9UJU6

Core Product Specifications and Parameters

Biological Background: DBNL Function and Localization

• DBNL (Drebrin-like protein), also known as HIP-55, SH3P7, or ABP1, is an adapter protein encoded by the DBNL gene. It exists in multiple isoforms due to alternative splicing.
• It binds F-actin and dynamin-1 (DNM1) and plays a role in receptor-mediated endocytosis and actin cytoskeleton reorganization. It does not bind G-actin and does not promote actin polymerization on its own.
• DBNL is involved in the formation of cell projections, such as neurites, and contributes to neuron morphogenesis and synapse formation via interactions with WASL and COBL.
• Required for the assembly of organized podosome rosettes, actin-rich structures important for adhesion and invasion.
• In leukocytes, it acts as a common effector of antigen receptor-signaling pathways and is a key component of the immunological synapse, bridging T-cell receptor (TCR) signaling to the actin cytoskeleton and gene activation.
• Subcellularly, DBNL localizes to the cytoplasm, cytoskeleton, cell cortex, lamellipodia, ruffles, podosomes, clathrin-coated vesicles, early endosomes, Golgi apparatus, and postsynaptic densities.
• The protein is regulated by acetylation and phosphorylation, and it is expressed in tissues with prominent roles in the nervous and immune systems.

Experimental Guidance and Technical Tips

• The antibody is raised against a recombinant protein spanning amino acids 131-430 of human DBNL. This region covers a substantial portion of the protein, likely including conserved motifs; thus, it may recognize endogenous DBNL across species due to sequence homology.
• For Western blotting (WB), a band near 48 kDa is anticipated. Optimize denaturing conditions and sample preparation; validate in relevant cell lines or tissues known to express DBNL (e.g., immune cells, neuronal cells).
• In ELISA applications, the polyclonal nature can provide broad epitope recognition. Titrate antibody concentrations and consider blocking solutions to reduce background.
• Since DBNL is involved in actin dynamics and endocytosis, treatments that modulate the cytoskeleton may alter its localization or abundance, providing useful experimental controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop sourcing solution for IVD raw materials. Beyond this anti-DBNL polyclonal antibody, we offer tailored procurement of validated antibody pairs, recombinant proteins, and ancillary reagents for immunology and neurobiology research. Let us handle your supply chain from concept to clinic, ensuring timely access to high-quality raw materials for your assay development needs.