Anti-DBI Rabbit Polyclonal Antibody for WB, IP, ELISA - P07108

Core Product Specifications and Parameters

Biological Background: DBI Function and Localization

• DBI, also known as Acyl-CoA-binding protein (ACBP), Endozepine (EP), ACBD1, or CCK-RP, is a highly conserved lipid-binding protein encoded by the DBI gene.
• It binds medium- and long-chain acyl-CoA esters with submicromolar affinity, functioning as an intracellular acyl-CoA carrier that facilitates lipid metabolism and transport.
• DBI competitively displaces diazepam from the benzodiazepine recognition site on the GABA type A receptor, suggesting a neuromodulatory role as an endogenous neuropeptide (endozepine).
• Subcellularly, DBI is localized to the endoplasmic reticulum and Golgi apparatus, consistent with its roles in lipid trafficking and neuropeptide processing.
• Alternative splicing and promoter usage generate three isoforms: isoform 1 is ubiquitous at moderate levels, isoform 2 is highly expressed in liver and adipose tissue, and isoform 3 shows strong expression in adipose tissue and heart.
• Post-translational modifications include acetylation, hydroxylation, and phosphorylation, as identified by proteomics, which may regulate its activity or localization.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-104 of human DBI (isoform 1); this region is conserved across isoforms, so the antibody may detect multiple isoforms—validate specificity in your sample system.
• For Western blotting, expect a band at 10-12 kDa under reducing conditions; use appropriate loading controls and consider optimizing blocking and antibody dilution based on your cell/tissue lysate.
• In immunoprecipitation, the antibody may enrich DBI and its interacting proteins; include negative controls (e.g., rabbit IgG) and validate by Western blot or mass spectrometry.
• For ELISA applications, check compatibility with your coating buffer and detection system; pair with a suitable DBI standard curve for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a comprehensive portfolio of raw materials, including validated antibody pairs, recombinant proteins, and bulk reagents. This anti-DBI polyclonal antibody addresses research needs in metabolic disorders and neuroscience, supporting assay developers with reliable detection of a key lipid carrier and neuropeptide. From initial feasibility to clinical validation, our consulting and sourcing services provide a single-point solution to accelerate your diagnostic projects.