Anti-DBI Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P31786

Core Product Specifications and Parameters

Biological Background: DBI Function and Localization

• DBI (Diazepam-binding inhibitor), also known as Acyl-CoA-binding protein (ACBP), endozepine (EP), or ACBD1, is a ~10 kDa protein encoded by the Dbi gene in mice.
• It binds medium- and long-chain acyl-CoA esters with very high affinity and is proposed to function as an intracellular carrier of acyl-CoA esters.
• DBI also acts as a neuropeptide: it can displace diazepam from the benzodiazepine recognition site on the GABA type A receptor, potentially modulating GABAergic neurotransmission.
• Subcellular localization is mainly to the endoplasmic reticulum and Golgi apparatus, consistent with its role in lipid metabolism and trafficking.
• The protein undergoes post-translational modifications including acetylation, hydroxylation, and phosphorylation, which may regulate its activity or localization.
• As a lipid-binding protein, DBI is involved in intracellular acyl-CoA transport, impacting processes such as fatty acid oxidation, membrane synthesis, and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the N-terminal 1-87 amino acids of mouse DBI (NP_031856.1). This region is well-conserved; the antibody is expected to recognize the full-length endogenous protein.
• For Western blot, a band at 10-12 kDa is expected under reducing conditions. Validate with appropriate positive controls (mouse or rat tissue lysates known to express DBI).
• In immunofluorescence and immunohistochemistry (paraffin sections), DBI localizes to the ER and Golgi apparatus. Co‑staining with organelle markers is recommended to confirm specificity.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a monoclonal antibody for sandwich ELISA after validation in your assay system.
• Since DBI is ubiquitously expressed, most tissues should be positive, but consider testing a range of samples to establish baseline expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting diagnostic developers from concept to clinic. As a trusted supplier, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This DBI polyclonal antibody is suitable for research into lipid metabolism and neurobiology; contact us for matched antigen or complementary reagents to accelerate your assay development.