Anti-DBI Rabbit Monoclonal Antibody for WB and ELISA - P07108

Core Product Specifications and Parameters

Biological Background: DBI Function and Localization

• DBI encodes acyl-CoA-binding protein, also known as diazepam-binding inhibitor (DBI) or endozepine (EP), a highly conserved protein involved in lipid metabolism and neurotransmission.
• It binds medium- and long-chain acyl-CoA esters with very high affinity, potentially acting as an intracellular carrier of acyl-CoA esters.
• DBI can displace diazepam from the benzodiazepine recognition site on the GABAA receptor, suggesting a modulatory role in GABAergic signaling.
• Multiple isoforms are produced through alternative promoter usage and splicing: isoform 1 is ubiquitous with moderate expression, isoform 2 is highly expressed in liver and adipose tissue, and isoform 3 is strongly expressed in heart and adipose tissue.
• The protein predominantly localizes to the endoplasmic reticulum and Golgi apparatus, consistent with its role in lipid trafficking.
• Post-translational modifications include acetylation, hydroxylation, and phosphorylation, as identified by proteomics and other experimental approaches.
• The mature protein has a predicted mass of approximately 10 kDa, aligning with the observed 10–12 kDa band in Western blot analysis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-104 of human DBI, which corresponds to the full-length protein; therefore, the antibody is expected to recognize the native form.
• For Western blotting, DBI migrates at 10–12 kDa; use appropriate low-molecular-weight markers and a high-percentage polyacrylamide gel for optimal resolution.
• The antibody is validated for ELISA; consider pairing it with a suitable detection antibody when developing sandwich immunoassays.
• Given DBI's roles in lipid metabolism and neurobiology, validation in relevant sample systems (e.g., liver, adipose, or brain tissues/ cell lines) is recommended.
• To maintain antibody performance, avoid repeated freeze-thaw cycles and store according to the supplier’s recommendations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio operates as a comprehensive one-stop sourcing platform for IVD raw materials, empowering diagnostic manufacturers with reliable antibodies, antigens, and ancillary reagents across the development pipeline. This anti-DBI monoclonal antibody targets a key player in lipid metabolism and neuroscience research; we supply it in bulk along with complementary reagents such as recombinant proteins and validated antibody pairs. Whether you need rare target raw materials or technical consulting, CamelBio bridges the gap from concept to clinic.