Anti-DBH Rabbit Monoclonal Antibody for WB, IP, IF, IHC-P, ELISA - P09172

Core Product Specifications and Parameters

Biological Background: Dopamine Beta-Hydroxylase Function and Localization

• Dopamine beta-hydroxylase (DBH), also known as dopamine beta-monooxygenase, is a copper-containing monooxygenase that catalyzes the hydroxylation of dopamine to noradrenaline, a critical step in catecholamine neurotransmitter biosynthesis.
• The enzyme is localized in the lumen of secretory vesicles, including chromaffin granules of the adrenal medulla and large dense-core vesicles of sympathetic neurons, and can also be secreted into the extracellular space.
• DBH exists as a tetrameric glycoprotein with intermolecular disulfide bonds, contributing to its structural stability and enzymatic function.
• It requires copper ions and ascorbate (vitamin C) as cofactors for full catalytic activity, linking its function to cellular redox and metal homeostasis.
• Mutations in the DBH gene are associated with dopamine beta-hydroxylase deficiency, a rare autosomal recessive disorder characterized by orthostatic hypotension, ptosis, and noradrenaline deficiency.
• The protein has a predicted molecular weight of approximately 69 kDa, with extensive post-translational modifications including N-linked glycosylation.
• DBH is anchored to secretory vesicle membranes via a signal-anchor transmembrane segment, with the majority of the protein, including the catalytic domain, facing the vesicle lumen.
• Keywords from UniProt include 3D-structure, catecholamine biosynthesis, copper, disease variant, glycoprotein, and secreted, underscoring its multifaceted roles in neurobiology and human disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 388-617 of human DBH, a region predicted to be part of the catalytic domain, therefore the antibody is likely to recognize the mature, active form of the protein.
• For Western blotting (WB), consider optimizing sample preparation using reducing and denaturing conditions, and anticipate a band around 69 kDa in cell lysates or adrenal tissue extracts.
• This antibody is suitable for multiple applications including immunoprecipitation (IP), immunofluorescence (IF/ICC and IF-P), and immunohistochemistry (IHC-P); validate antibody dilutions empirically in the relevant sample system.
• Given cross-reactivity with mouse DBH, the antibody can be employed in transgenic or knock-out mouse models to confirm specificity; use appropriate positive and negative control tissues.
• For ELISA, consider coating with recombinant DBH protein or using a sandwich format with a paired capture/detection antibody to improve sensitivity and dynamic range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our DBH antibody portfolio supports research into catecholamine-related disorders, neurological biomarkers, and in vitro diagnostic assay development. Leverage our validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing capabilities to accelerate your project timelines.