Anti-DAZL Rabbit Polyclonal Antibody for WB, ELISA - Q92904

Core Product Specifications and Parameters

Biological Background: DAZL Function and Localization

• DAZL (Deleted in azoospermia-like) is an RNA-binding protein that shares homology with the DAZ gene family, and it is also known as DAZ-like autosomal (DAZLA) or SPGY-like-autosomal.
• It plays an essential role in gametogenesis in both males and females by acting as a key regulator of spermatogenesis.
• DAZL binds to the 3'-UTR of target mRNAs, recognizing GUU triplets, and thereby modulates translation of transcripts critical for germ cell development (By similarity).
• The protein is primarily localized in the cytoplasm but can also be found in the nucleus, consistent with its roles in mRNA metabolism.
• DAZL is specifically expressed in the testis, underscoring its specialized function in reproductive biology.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, contributing to its regulatory complexity.
• Keywords associated with DAZL include: Alternative splicing, Cytoplasm, Developmental protein, Differentiation, Nucleus, Phosphoprotein, RNA-binding, Spermatogenesis, Translation regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 186-295 of human DAZL; this region may be conserved across species, consistent with the observed cross-reactivity with mouse.
• For Western blot, the predicted molecular weight is 33 kDa; consider using appropriate loading controls and validate band specificity in the relevant sample system.
• For ELISA, ensure the antibody concentration is optimized for the assay format and sample type.
• Given its testis-specific expression, validate in human or mouse testis lysates for WB to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined procurement channel for high-quality IVD antibodies like this DAZL polyclonal antibody. Our portfolio covers reproductive biomarkers and developmental targets, supported by a network that can source validated antibody pairs, monoclonal alternatives, and bulk ancillary reagents. Whatever your raw-material needs, from early concept to clinical application, CamelBio is your dedicated sourcing partner.