Anti-DAZAP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96EP5

Core Product Specifications and Parameters

Biological Background: DAZAP1 Function and Localization

• DAZAP1 (Deleted in azoospermia-associated protein 1) is an RNA-binding protein encoded by the DAZAP1 gene in humans.
• The protein is proposed to be required during spermatogenesis, likely influencing RNA metabolism in germ cells.
• Subcellular localization includes both cytoplasm and nucleus, consistent with roles in RNA processing and transport.
• Expression is predominantly testis-specific, with lower levels in thymus and minimal expression in other tissues, indicating a highly specialized reproductive function.
• Post-translational modifications such as acetylation and methylation may regulate its activity or interactions.
• Alternative splicing generates multiple isoforms, potentially modulating function during differentiation and development.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 265-407) of human DAZAP1; antibody specificity should be confirmed using recombinant protein or knockdown controls.
• For Western blot, the expected band size is ~43 kDa; testis lysates are recommended as a positive control and reducing conditions should be used.
• In IHC-P, strong staining is anticipated in testis tissue due to high target expression; antigen retrieval and antibody titration should be optimized for each sample type.
• ELISA applications require careful titration and inclusion of positive/negative controls to ensure linearity and specificity.
• Predicted cross-reactivity with mouse and rat DAZAP1 is based on sequence homology; users should validate performance in their species and tissue of interest.
• All experimental conditions must be optimized by the end user according to their specific protocols and sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, technical services, and consulting to support every stage of diagnostic development. For reproductive biology and spermatogenesis research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with CamelBio to efficiently access high-quality reagents and accelerate your IVD workflow from concept to clinic.