Anti-Daxx Rabbit Polyclonal Antibody for WB and ELISA - Q9UER7

Core Product Specifications and Parameters

Biological Background: Daxx Function and Localization

• Daxx (Death domain-associated protein 6), also referred to as hDaxx, EAP1 (ETS1-associated protein 1), BING2, or DAP6, is a versatile nuclear and cytoplasmic protein involved in transcriptional regulation, apoptosis, and chromatin dynamics.
• Functions as a transcription corepressor by inhibiting the transcriptional activity of sumoylated factors such as PAX3, ETS1, and PAX5, often through recruitment to PML nuclear bodies.
• Acts as a histone chaperone that specifically facilitates replication-independent deposition of the histone variant H3.3, partnering with the chromatin remodeler ATRX to deposit H3.3 at pericentric repeats and telomeres.
• Mediates apoptosis signaling by linking death receptors (TNFRSF6/Fas and TGFBR2) to the JNK pathway via MAP3K5 (ASK1), though this mechanism may be cell-type specific and modulated by HSPB1/HSP27.
• Positively regulates heat shock factor HSF1 activity during stress responses, enhancing the cellular stress protein response. Related references: PMID:15016915
• Localizes to multiple subcellular compartments including the nucleoplasm, PML nuclear bodies, nucleolus, cytoplasm, and centromeric chromatin, reflecting its diverse functions.
• Exhibits antiviral restriction activity toward human cytomegalovirus (HCMV), implicating it in host defense mechanisms.
• Undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which likely modulate its interactions and functions.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole-cell lysates from human or mouse cell lines, as Daxx is ubiquitously expressed. The predicted molecular weight is 81 kDa, but actual migration may vary due to post-translational modifications.
• For ELISA, ensure the immunogen region (aa 175-400) is accessible in the native protein conformation if using sandwich ELISA; otherwise, direct coating with recombinant protein may be used.
• Given the polyclonal nature, this antibody may detect multiple isoforms or species variants; validate in the relevant experimental systems.
• If studying Daxx interactions or modifications, consider the use of appropriate inhibitors or stimulation conditions (e.g., stress induction for HSF1 pathway).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Daxx polyclonal antibody supports research in transcriptional regulation, apoptosis, and chromatin biology, and can be integrated into larger assay development projects. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to streamline your pipeline.