Anti-DARS2 Polyclonal Antibody for WB, IHC-P, ELISA - Q6PI48

Core Product Specifications and Parameters

Biological Background: DARS2 Function and Localization

• DARS2 encodes mitochondrial aspartyl-tRNA synthetase (AspRS), a member of the class-II aminoacyl-tRNA synthetase family critical for mitochondrial protein synthesis.
• Catalyzes the two-step attachment of aspartate to tRNA(Asp): first activating aspartate with ATP to form Asp-AMP, then transferring aspartate to the tRNA acceptor end.
• Localizes primarily to the mitochondrial matrix and may associate with the mitochondrial membrane, reflecting its role in mitochondrial translation.
• Alternate names include LBSL, ASPRS, mtAspRS, and MT-ASPRS; the protein is the mitochondrial counterpart of cytoplasmic DARS.
• Mutations in DARS2 cause leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL), linking it to neurodegeneration and Charcot-Marie-Tooth disease.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination have been identified, potentially regulating enzyme activity or stability.
• The protein is also annotated with 3D-structure, ATP-binding, and isopeptide bond, highlighting its conserved catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 406–645), which includes part of the catalytic domain; confirm the detected band at ~74 kDa in your sample type and consider the possibility of alternative splicing or processing.
• For Western blot, optimize antibody dilution (e.g., 1:500–1:2000) and use mitochondrial-enriched lysates to enhance signal, given the mitochondrial localization.
• In IHC-P, test antigen retrieval conditions (e.g., citrate buffer pH 6.0) and include a no-primary control to assess specificity.
• For ELISA, pair with an appropriate enzyme-conjugated secondary antibody and validate linearity using recombinant DARS2 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined one-stop platform for IVD raw-material procurement, spanning concept to clinic. Our DARS2 antibody supports mitochondrial translation and neurodegenerative disease research; we can supply matched antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets like mitochondrial aminoacyl-tRNA synthetases, CamelBio leverages its extensive network to accelerate your raw-material sourcing.