Anti-DARPP32 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UD71

Core Product Specifications and Parameters

Biological Background: DARPP32 Function and Localization

• DARPP32, also known as Protein phosphatase 1 regulatory subunit 1B (PPP1R1B), is a dopamine- and cAMP-regulated neuronal phosphoprotein.
• It functions as an inhibitor of protein phosphatase 1 (PP1), modulating downstream signaling cascades.
• Subcellular localization is primarily cytoplasmic.
• Post-translational modifications include phosphorylation and acetylation, consistent with its role as a phosphoprotein.
• Alternative splicing generates multiple isoforms, adding functional diversity.
• Classified under protein phosphatase inhibitors and has been identified in proteomics screens.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–116). For Western blot, a band near 23 kDa is expected; validate in the relevant sample system.
• For immunofluorescence/ICC, appropriate fixation and permeabilization conditions should be optimized.
• ELISA performance should be verified with recombinant protein or native samples, considering potential epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This DARPP32 polyclonal antibody supports neuroscience and signal transduction research, and we can assist with complementary reagents and technical consultation to streamline your assay development from concept to clinic.