Anti-DARPP32 Monoclonal Antibody for WB, IF/ICC, IF-F, ELISA - Q9UD71

Core Product Specifications and Parameters

Biological Background: DARPP32 Function and Localization

• DARPP32, also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B) or dopamine- and cAMP-regulated neuronal phosphoprotein, is a key regulator of signal transduction in the brain.
• It functions as a potent inhibitor of protein phosphatase 1 (PP1), thereby modulating the phosphorylation state of downstream targets.
• DARPP32 is predominantly localized to the cytoplasm (source: UniProt Q9UD71).
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which regulate its activity.
• Multiple isoforms exist due to alternative splicing.
• DARPP32 plays a critical role in dopamine signaling pathways, integrating dopaminergic and glutamatergic inputs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 100-200 of human DARPP32; consider this region when assessing species cross-reactivity based on sequence alignment.
• For Western blot (WB), the predicted molecular weight is ~23 kDa; validate band size in the relevant tissue or cell lysate.
• For immunofluorescence (IF/ICC and IF-F), optimize fixation and permeabilization conditions to preserve antigenicity.
• ELISA applications may require optimization of coating buffer and antibody concentration; perform a standard curve with known antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined, one-stop solution for IVD raw material sourcing. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. For rare targets like DARPP32, CamelBio leverages global supply networks to ensure rapid access to critical raw materials.