Anti-DAPP1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UN19

Core Product Specifications and Parameters

Biological Background: DAPP1 Function and Localization

• DAPP1 (also known as BAM32) is a B lymphocyte adapter protein of approximately 32 kDa.
• It functions as a dual adapter for phosphotyrosine and 3-phosphoinositides, linking B-cell antigen receptor (BCR) signaling to downstream phosphoinositide 3-kinase (PI3K) pathways.
• The protein contains an SH2 domain and a pleckstrin homology (PH) domain, enabling membrane recruitment and protein interaction.
• DAPP1 is predominantly localized in the cytoplasm and can associate with the plasma membrane upon activation.
• Tissue expression is highest in placenta and lung, with lower levels in brain, heart, kidney, liver, pancreas, and skeletal muscle.
• It is specifically expressed in B-lymphocytes and not detected in T-lymphocytes or non-hematopoietic cells.
• Post-translational modifications include phosphorylation, consistent with its role in signaling.
• Alternative splicing generates multiple transcript variants, potentially modulating its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100–273, a central region of DAPP1; this design may enhance detection of full-length DAPP1 while requiring validation for isoform specificity.
• For Western blotting, DAPP1 is a cytoplasmic and membrane-associated protein; use appropriate lysis buffers and consider low-speed centrifugation to retain membrane fractions.
• In immunofluorescence, membrane localization may be stimulus-dependent; fixation and permeabilization conditions should be optimized to visualize both cytoplasmic and membrane pools.
• For ELISA, the peptide immunogen may serve as a positive control; however, titration and cross-reactivity testing against similar SH2/PH domain proteins is advised.
• DAPP1 expression is B-cell specific; validate antibody performance in B-lymphocyte lines (e.g., Ramos, Raji) rather than T-cell lines to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, supporting projects from early development to clinical application. For B-cell signaling research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare targets like DAPP1. From procurement to technical consultation, CamelBio accelerates your assay development with reliable supply solutions.