Anti-DAPK3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43293

Core Product Specifications and Parameters

Biological Background: DAPK3 Function and Localization

• DAPK3 (Death-associated protein kinase 3), also known as ZIPK or Dlk, is a serine/threonine kinase that plays a central role in regulating apoptosis, autophagy, transcription, translation, and actin cytoskeleton dynamics.
• Through phosphorylation of myosin light chains (MYL12B, MYL9) and the myosin phosphatase target subunit (PPP1R12A), DAPK3 modulates smooth muscle contraction and actin stress fiber organization in non-muscle cells.
• The kinase participates in both caspase-dependent (type I) and caspase-independent (type II) cell death pathways, and its overexpression can drive apoptosis or autophagic cell death depending on cellular context.
• DAPK3 localizes to multiple subcellular compartments, including the nucleus, PML bodies, centrosomes, centromeres, spindle, midbody, and the actin cytoskeleton, reflecting its diverse functional roles.
• It positively regulates canonical Wnt/β-catenin signaling via interaction with NLK and TCF7L2, and enhances androgen receptor-mediated transcription, linking it to developmental and hormonal pathways.
• Under interferon-gamma stimulation, DAPK3 phosphorylates ribosomal protein RPL13A, promoting its release from ribosomes and engagement in the GAIT complex to inhibit translation of specific transcripts.
• The protein is widely expressed across tissues, with isoform 1 and isoform 2 notably present in bladder smooth muscle, and is classified as a potential tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human DAPK3; thus, the antibody is expected to recognize epitopes within this domain.
• A band at approximately 53 kDa should be observed by Western blotting, consistent with the predicted molecular weight of DAPK3. Verify detection in lysates from appropriate cell lines or tissues expressing the target.
• For immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), fixation and antigen retrieval conditions may influence staining; titrate the antibody and include appropriate controls (e.g., knockdown or knockout samples) to confirm specificity.
• The antibody is reported to cross-react with mouse and rat DAPK3, likely due to sequence conservation in the immunogen region; however, users should validate reactivity in their specific experimental systems.
• As a polyclonal antibody, lot-to-lot variability may occur. It is advisable to titrate each new lot and consider using blocking peptides for competition assays if non-specific binding is suspected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a seamless, one-stop procurement channel for IVD raw materials like this DAPK3 rabbit polyclonal antibody, tailored for apoptosis, autophagy, and cytoskeleton research. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing—all designed to support your workflow from concept to clinic.