Anti-DAP3 Rabbit Polyclonal Antibody for WB, ELISA - P51398

Core Product Specifications and Parameters

Biological Background: DAP3 Function and Localization

• Encoded by the DAP3/MRPS29 gene, the protein is known as small ribosomal subunit protein mS29 (also MRP-S29, S29mt) and was originally identified as death-associated protein 3 (DAP3). Alternative splicing produces multiple isoforms; UniProt lists the canonical isoform at 45566 Da.
• As a component of the mitochondrial 28S small ribosomal subunit, DAP3 is essential for the translation of mitochondrial mRNAs, thereby supporting oxidative phosphorylation. Related references: PMID:39701103
• DAP3 mediates interferon-gamma-induced cell death, establishing a direct link between mitochondrial function and apoptosis. Related references: PMID:7499268
• In vitro experiments demonstrate intrinsic GTPase activity, pointing to a possible role in ribosomal dynamic processes or pro-apoptotic signaling. Related references: PMID:39701103
• Subcellularly, DAP3 localizes to the mitochondrion; it is ubiquitously expressed across tissues, consistent with its housekeeping role in mitochondrial translation.
• Post-translational modifications include acetylation, and the protein is subject to alternative splicing. Known disease associations involve deafness (non-syndromic) and potential tumor-related variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central to C-terminal region (aa 159–398), which may detect multiple DAP3 isoforms; confirm specificity in the relevant cell systems.
• For Western blotting, consider a starting dilution range (e.g., 1:500–1:2000) with whole-cell or mitochondrial-enriched lysates from human, mouse, or rat samples. The observed band is around 46 kDa.
• In ELISA, titration of both antibody and antigen is recommended to establish optimal working concentrations. Polyclonal reactivity may vary between batches; include appropriate controls.

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CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. For mitochondrial biology and apoptosis research, we can source rare-target raw materials and provide supply-chain support to accelerate your project from concept to clinic.