Anti-DAP Kinase 1 (DAPK1) Polyclonal Antibody for WB, IHC-P, ELISA - P53355

Core Product Specifications and Parameters

Biological Background: DAP Kinase 1 (DAPK1) Function and Localization

• DAPK1 is a calcium/calmodulin-dependent serine/threonine kinase that integrates multiple cellular signaling pathways, governing cell survival, apoptosis, and autophagy.
• It regulates both caspase-dependent type I apoptotic death and caspase-independent type II autophagic cell death, depending on cellular context.
• DAPK1 phosphorylates a diverse array of substrates—including PIN1, TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3—modulating nuclear localization, cytoskeletal dynamics, vesicle trafficking, and metabolic signaling.
• In stroke, DAPK1 acts as an NMDA receptor signaling amplifier, phosphorylating GRINB at Ser-1303 to drive injurious Ca2+ influx and irreversible neuronal death.
• Isoform 2, generated by alternative splicing, cannot induce apoptosis but promotes membrane blebbing; it is expressed in normal intestinal tissue and colorectal carcinomas.
• Subcellular localization is predominantly cytoplasmic and associated with the cytoskeleton, consistent with its roles in remodeling and signaling.
• The protein features a multidomain architecture that includes a kinase domain, a calmodulin-binding domain, ankyrin repeats, and a death domain, underpinning its function in cell death regulation.

Experimental Guidance and Technical Tips

• For WB, a specific band at approximately 160 kDa is expected in lysates from human, mouse, or rat samples; confirm specificity using appropriate positive and negative controls.
• In IHC-P, optimize antigen retrieval and antibody dilution; consider known expression patterns (e.g., brain, intestinal epithelium) for validation.
• For ELISA, the antibody can be used as a capture or detection reagent; empirical determination of coating concentration and pairing partners is recommended.
• Because the immunogen maps to the C-terminal region (aa 1141-1430), verify detection of isoform 2 in relevant experimental systems if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories worldwide with a comprehensive portfolio of IVD raw materials, including high-quality antibodies targeting key regulators like DAPK1. Whether you need validated antibody pairs, optimized polyclonal or monoclonal reagents, bulk ancillary products, or assistance sourcing rare-target raw materials for apoptosis and autophagy studies, CamelBio provides end-to-end procurement and technical consultation to accelerate your assay development.