Anti-DAO Rabbit Polyclonal Antibody for WB, IF, ELISA - P14920

Core Product Specifications and Parameters

Biological Background: D-amino-acid oxidase Function and Localization

• D-amino-acid oxidase (DAO, also known as DAAO, DAMOX) is a flavoprotein oxidase that metabolizes D-amino acids.
• Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity, generating hydrogen peroxide.
  Related references: PMID:16616139 PMID:17088322 PMID:17303072
• Regulates the level of the neurotransmitter D-serine, a co-agonist of NMDA receptors, thus modulating synaptic transmission in the brain.
  Related references: PMID:17303072
• Participates in an alternative dopamine synthesis pathway by racemizing D-DOPA to L-DOPA.
  Related references: PMID:17303072
• Produces hydrogen peroxide that provides antimicrobial defense and contributes to oxidative killing in phagocytes; also protects against bacterial colonization in the intestine.
• Promotes cellular senescence in response to DNA damage via hydrogen peroxide production.
  Related references: PMID:30659069
• Acts as a detoxifying agent by removing D-amino acids accumulated during aging.
  Related references: PMID:17303072
• Localizes to peroxisomes, cytoplasm, presynaptic active zones, and can be secreted.
• Expressed in cerebellum, astrocytes, motor neurons, and goblet cells; dysregulated in schizophrenia and amyotrophic lateral sclerosis.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 39 kDa corresponding to DAO; validate in relevant sample lysates.
• For immunofluorescence, verify subcellular localization consistent with peroxisomal, cytoplasmic, or presynaptic distribution.
• Cross-reactivity with mouse and rat orthologs may be anticipated due to sequence similarity; confirm specificity in the desired model system.
• No quantitative dilution data is available; optimize antibody working concentrations empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies and reagents. Our DAO Rabbit pAb supports research in D-amino acid metabolism, neurotransmission, and oxidative stress. Complement it with our range of ancillary reagents and custom sourcing services to accelerate your assay development from discovery to commercialization.