Anti-DAO Monoclonal Antibody for WB, ELISA - P14920

Core Product Specifications and Parameters

Biological Background: DAO Function and Localization

• DAO is a peroxisomal flavoprotein (FAD-containing oxidoreductase) that catalyzes the oxidative deamination of D-amino acids with wide substrate specificity.
  Related references: PMID:16616139 PMID:17088322 PMID:17303072
• It regulates synaptic transmission by controlling levels of the NMDA receptor co-agonist D-serine in the brain.
  Related references: PMID:17303072
• DAO initiates the racemization of D-DOPA to L-DOPA, linking it to dopamine biosynthesis.
  Related references: PMID:17303072
• The hydrogen peroxide byproduct of its reaction contributes to host defense via oxidative killing and can promote DNA damage-induced cellular senescence.
  Related references: PMID:30659069
• It localizes to peroxisomes, cytosol, presynaptic active zones, and is also secreted; it is expressed in cerebellum, astrocytes, motor neurons, and intestinal goblet cells, with altered expression in schizophrenia and ALS.
  Related references: PMID:17880399 PMID:18544534 PMID:18560437 PMID:24138986 PMID:34041270 PMID:27670111
• Post-translational modifications include phosphorylation and S-nitrosylation; it may also be proteolytically processed into antimicrobial peptides.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human DAO; this region is well conserved across human, mouse, and rat, supporting the observed cross-reactivity. Verify sequence alignment for other species.
• For Western blotting, the predicted molecular weight of DAO is approximately 39 kDa. Optimize sample preparation (e.g., reducing conditions, tissue type) and antibody concentration to detect the target band, especially in tissues with low endogenous expression.
• In ELISA applications, test the antibody in your specific assay format; it may be used as a capture or detection antibody after appropriate validation and pairing.
• Since DAO is a peroxisomal enzyme, consider using appropriate subcellular fractionation or marker controls in localization studies.

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