Anti-DAG1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q14118

Core Product Specifications and Parameters

Biological Background: DAG1 Function and Localization

• DAG1 is cleaved into alpha and beta dystroglycan; alpha-dystroglycan is a heavily glycosylated extracellular receptor, while beta-dystroglycan is a transmembrane protein linking to the cytoskeleton.
• Alpha-dystroglycan binds laminins, agrin, perlecan, and pikachurin, mediating basement membrane assembly, cell adhesion, and signaling through pathways such as PI3K/AKT and Src/Rac1/JNK.
• Beta-dystroglycan anchors the dystrophin-glycoprotein complex to dystrophin or utrophin, connecting the extracellular matrix to the actin cytoskeleton.
• At the neuromuscular junction, it clusters acetylcholine receptors and anchors acetylcholinesterase via perlecan binding.
• In cartilage, agrin binding upregulates SOX9, a key transcription factor for chondrocyte differentiation. Related references: PMID:26290588
• Serves as a receptor for Lassa virus, lymphocytic choriomeningitis virus, and other class C arenaviruses. Related references: PMID:16254364, PMID:17360738, PMID:19324387
• Mediates Schwann cell adhesion and internalization of Mycobacterium leprae. Related references: PMID:9851927
• Localizes to the extracellular matrix, basement membrane, sarcolemma, synapse, cytoplasm, and nucleus; expressed in a variety of fetal and adult tissues, including keratinocytes and fibroblasts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 776–895) of human DAG1, likely yielding reactivity with both alpha- and beta-dystroglycan isoforms. Consider verifying reactivity in your species and tissue of interest.
• For Western blot, the intact precursor is ~97 kDa, but alpha-dystroglycan often appears as a broad band due to extensive glycosylation. A ~43 kDa beta-dystroglycan band may also be detected.
• In IF/ICC and IP, validate fixation/permeabilization and antibody concentration, as epitope accessibility may be influenced by glycosylation and subcellular localization.
• ELISA is suitable for detecting recombinant DAG1 or native protein in lysates; include appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. For dystroglycan and extracellular matrix research, we offer a reliable supply of polyclonal and monoclonal antibodies, bulk production support, and custom sourcing for rare targets. Let CamelBio accelerate your assay development from concept to clinic.