Anti-DACT3 Rabbit Polyclonal Antibody for WB and ELISA - Q96B18

Core Product Specifications and Parameters

Biological Background: DACT3 Function and Localization

• DACT3, also known as Dapper homolog 3, antagonist of beta-catenin Dapper homolog 3, or arginine-rich region 1 (RRR1) protein, is a member of the Dapper antagonist of catenin (DACT) family.
• The protein is involved in the regulation of intracellular signaling pathways during development, with a specific role in modulating both canonical and non-canonical Wnt signaling via interaction with Dishevelled (DSH) family proteins.
• DACT3 contains coiled-coil domains (UniProt keyword), which likely mediate protein-protein interactions necessary for its function in signal transduction complexes.
• It is annotated as a phosphoprotein and a methylation substrate, suggesting its activity may be dynamically regulated by post-translational modifications.
• The protein is part of the reference proteome for Homo sapiens, indicating its use as a representative sequence in proteomics studies.
• While DACT3 is implicated in developmental processes, its precise tissue distribution and subcellular localization remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (amino acids 1-160) of human DACT3, which may confer specificity for this isoform. Validate reactivity in your target species and sample type before critical experiments.
• For Western blotting, the predicted molecular weight is approximately 65 kDa, but actual migration may vary depending on post-translational modifications and gel conditions. Include appropriate positive and negative controls.
• Cross-reactivity with mouse and rat is expected based on supplier data, making this antibody suitable for species-comparative studies. Consider performing a titration series to optimize concentration for your specific application.
• For ELISA, ensure the recombinant immunogen or a validated native protein source is used as the capture antigen to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering a reliable supply of validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Researchers investigating Wnt signaling and developmental pathways can leverage our DACT3 antibody to accelerate assay development, with access to rare target raw-material sourcing backed by technical consultation. We support your journey from concept to clinic with one-stop procurement convenience.