Anti-DACH1 Polyclonal Antibody for WB, ELISA - Q9UI36

Core Product Specifications and Parameters

Biological Background: DACH1 Function and Localization

• DACH1 is a transcription factor that plays a critical role in organogenesis and developmental processes.
• It acts as a coregulator of SIX family proteins (SIX1, SIX6, SIX5), switching between corepressor and coactivator functions depending on the binding partner (e.g., EYA3 or CREBBP).
• DACH1 inhibits TGF-beta signaling by interacting with SMAD4 and the corepressor NCOR1.
• The protein binds to chromatin DNA via its DACHbox-N domain, directly regulating gene expression.
• It is localized to the nucleus where it exerts its transcriptional regulatory functions.
• Multiple isoforms are generated through alternative splicing, with tissue-specific expression patterns (e.g., isoform 2 in brain, heart, kidney; isoform 3 in liver and heart; isoform 4 in spleen).
• DACH1 is widely expressed, highlighting its fundamental role in cellular regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 450-570 of human DACH1, located within the protein; consider this when designing detection protocols.
• For Western blot (WB), the predicted molecular weight is approximately 79 kDa, but alternative splicing may produce isoforms of varying sizes; validate with appropriate controls.
• For ELISA, use standard direct or indirect ELISA protocols; the antibody may be used for antigen capture or detection after proper titration.
• Since the antibody is raised in rabbit, use an anti-rabbit secondary antibody for detection; ensure cross-reactivity with your detection system is minimal.
• Cross-reactivity has been tested with human, mouse, and rat samples; if using other species, verify reactivity empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with validated IVD raw materials like this DACH1 polyclonal antibody, essential for developmental biology and transcription regulation studies. Our supply portfolio also includes optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents, all sourced under rigorous quality standards. Whether you need standard catalog products or specialized rare-target raw materials, CamelBio offers comprehensive support from concept to clinical application.