Anti-DAB2 Rabbit Polyclonal Antibody for WB, FC, ELISA - P98082

Core Product Specifications and Parameters

Biological Background: DAB2 Function and Localization

• DAB2 (Disabled homolog 2) is an adapter protein also known as adaptor molecule disabled-2, DOC-2, and differentially expressed in ovarian carcinoma 2.
• Functions as a clathrin-associated sorting protein (CLASP) essential for clathrin-mediated endocytosis; binds clathrin and PtdIns(4,5)P2 to recruit cargo containing NPXY motifs (e.g., LDL receptor) to coated pits.
• Involved in TGF-beta receptor signaling, facilitating SMAD2 phosphorylation; mediates TGF-beta-stimulated JNK activation and may inhibit canonical Wnt/beta-catenin signaling by stabilizing the destruction complex and sequestering LRP6.
• Localizes to the cytoplasm, associated with clathrin-coated pits and vesicles at the membrane.
• Expressed in ovarian surface epithelial cells, breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung, placenta, brain stem, heart, small intestine, and kidney proximal tubular cells at the protein level.
• Subject to acetylation and phosphorylation; involved in apoptosis, differentiation, development, endocytosis, protein transport, and Wnt signaling; acts as a tumor suppressor.
• Required for visceral endoderm formation, cell positioning, and epithelial-to-mesenchymal transition during embryogenesis; may also promote myeloid cell differentiation and macrophage adhesion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 340-580 of human DAB2; this region may contribute to the antibody's specific recognition profile.
• For Western blotting, the predicted molecular weight of ~82 kDa should guide gel percentage selection and transfer conditions.
• As a polyclonal antibody, it may detect multiple bands if splice variants or degradation products are present; validate in a relevant sample system.
• For flow cytometry, consider permeabilization due to cytoplasmic localization of DAB2.
• In ELISA, ensure proper coating with the immunogen peptide or recombinant DAB2 protein, and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents and customized monoclonal/polyclonal antibody development. For researchers targeting DAB2, a key adapter in endocytosis, TGF-beta/Wnt signaling, and tumor suppression, CamelBio can assist in selecting appropriate antibody formats and supplying related reagents to accelerate your diagnostic assay development.