Anti-DAB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75553

Core Product Specifications and Parameters

Biological Background: DAB1 Function and Localization

• DAB1 (Disabled homolog 1) serves as a key signaling adapter in the reelin-mediated pathway, essential for the migration and proper positioning of postmitotic neurons during brain development.
• Upon reelin binding to its receptor, DAB1 transduces intracellular signals by recruiting downstream effectors through its phosphotyrosine residues and PID (phosphotyrosine interaction domain).
• DAB1 is primarily localized in the cytoplasm, where it interacts with signaling complexes.
• It is predominantly expressed in the brain, supporting its critical role in central nervous system development.
• The protein is subject to multiple post-translational modifications, including phosphorylation (classified as a phosphoprotein) and ubiquitination (Ubl conjugation), which regulate its activity and stability.
• Alternative splicing generates multiple DAB1 isoforms, potentially contributing to functional diversity.
• Mutations and dysregulation of DAB1 are associated with neurodegenerative conditions, notably spinocerebellar ataxia type 37 (SCA37), highlighting its importance in neuronal maintenance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-185) of human DAB1; the antibody is expected to recognize full-length DAB1 and possibly certain isoforms.
• For Western blot (WB), consider using brain tissue lysates or neuronal cell lines from human, mouse, or rat, and validate the signal around the predicted 64 kDa band.
• Immunofluorescence/immunocytochemistry (IF/ICC) may require optimization of fixation and permeabilization conditions for cytoplasmic targets; include appropriate controls.
• ELISA performance can be assessed using recombinant DAB1 protein or cell lysates; consider titration to determine optimal coating and detection conditions.
• Cross-reactivity with mouse and rat DAB1 makes this antibody suitable for comparative studies across species, but always validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of critical IVD raw materials for diagnostic and research communities. This anti-DAB1 antibody supports investigations into reelin signaling and neurodevelopmental pathways. Our portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—empowering your assay development from concept to clinic.