Anti-DAAM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q86T65

Core Product Specifications and Parameters

Biological Background: DAAM2 Function and Localization

• DAAM2 (Disheveled-associated activator of morphogenesis 2) is a 123 kDa formin homology protein encoded by the DAAM2 gene (also known as KIAA0381), and functions as a key regulator of both canonical and non-canonical Wnt signaling pathways.
• Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1) to promote dorsal spinal cord patterning by facilitating Disheveled complex aggregation and Wnt signalosome formation; also inhibits oligodendrocyte differentiation via interaction with PIP5K1A.
• Collaborates with DAAM1 in myocardial maturation and sarcomere assembly, essential for proper heart development.
• Plays a critical role in establishing left/right asymmetry during gut development: downstream of PITX2, it mediates WNT5A-driven non-canonical Wnt signaling to modulate cadherin-based junctions and mesenchymal condensation.
• Regulates actin nucleation and elongation, filopodia formation, and podocyte migration, with implications in kidney biology. Related references: PMID:33232676
• Exhibits broad tissue expression, notably in kidney glomeruli, and is associated with nephrotic syndrome type 24 (NPHS24) when mutated.
• Subject to alternative splicing and post-translational phosphorylation; contains a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-86 of human DAAM2) corresponds to the N-terminal region; consider its conservation across species and isoforms when designing detection strategies.
• For Western blot (WB), expect a band around 123 kDa; validate in relevant cell lines or tissue extracts, as expression may vary.
• This antibody is suitable for immunofluorescence (IF/ICC) and ELISA; titrate and optimize concentrations for each application and sample type.
• Due to DAAM2's role in Wnt signaling, appropriate pathway activation controls may be necessary for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier provider of IVD raw materials, offering one-stop access to validated antibodies, bulk reagents, and rare target sourcing for diagnostic development. For DAAM2-focused Wnt signaling and nephrology research, our supply capabilities include optimized polyclonal antibodies and comprehensive technical support to streamline your assay development from concept to clinic.