Anti-D2HGDH Polyclonal Antibody for WB and ELISA - Q8N465

Core Product Specifications and Parameters

Biological Background: D2HGDH Function and Localization

• D2HGDH (D-2-hydroxyglutarate dehydrogenase) is a mitochondrial enzyme that catalyzes the oxidation of D-2-hydroxyglutarate to α-ketoglutarate, a critical step in the metabolism of D-2-hydroxyacids. Related references: PMID:15070399 PMID:15609246 PMID:16037974
• The enzyme also accepts D-malate and D-lactate as substrates, exhibiting high activity toward D-malate but very weak activity toward D-lactate. Related references: PMID:33431826
• D2HGDH is a flavoprotein that requires FAD and zinc ion as cofactors for its catalytic activity.
• It is localized to the mitochondrial matrix, with an N-terminal transit peptide directing its import into the organelle.
• Alternative splicing generates multiple transcript variants, and the protein is readily identified in proteomics reference datasets.
• Mutations in the D2HGDH gene are associated with D-2-hydroxyglutaric aciduria, a severe neurometabolic disorder, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (amino acids 322–521), so this polyclonal antibody is likely to detect both native and denatured D2HGDH.
• For Western blot, load 20–30 µg of reducing-denatured lysate from human, mouse, or rat samples and look for a band at approximately 56 kDa. Optimize the primary antibody dilution empirically.
• In ELISA, the antibody may be used as either a capture or detection reagent. Perform checkerboard titrations with your antigen to determine optimal working concentrations.
• Because lot-to-lot variability can occur with polyclonal antibodies, include appropriate positive and negative controls in every experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, providing high-quality antibodies for metabolic targets such as D2HGDH, which is relevant in cancer metabolism and mitochondrial disease research. We offer off-the-shelf and custom solutions, including validated antibody pairs, bulk ancillary reagents, and sourcing for difficult-to-find targets—streamlining your assay development from concept to clinic.