Anti-δ-Catenin/p120 Catenin Rabbit Polyclonal Antibody for WB and ELISA - O60716

Core Product Specifications and Parameters

Biological Background: δ-Catenin/p120 Catenin Function and Localization

• δ-Catenin/p120 Catenin (Catenin delta-1) is a key regulator of cell-cell adhesion that associates with C-, E- and N-cadherins, critical for their surface stability. Related references: PMID:14610055, PMID:20371349
• It promotes localization and retention of DSG3 at cell-cell junctions through direct interaction. Related references: PMID:18343367
• Beyond adhesion, it regulates gene transcription via transcription factors such as ZBTB33/Kaiso2 and GLIS2, and modulates Rho family GTPase activity affecting cytoskeletal dynamics. Related references: PMID:10207085, PMID:20371349
• Implicated in cell transformation by SRC and ligand-induced receptor signaling through EGF, PDGF, CSF-1 and ERBB2 receptors. Related references: PMID:17344476
• Subcellular localization includes adherens junctions, cytoplasm, nucleus, and cell membrane, reflecting its multifaceted roles.
• Tissue-specific expression: vascular endothelium; melanocytes and melanoma cells express long isoform 1A, while keratinocytes express shorter isoforms; isoform 4A is frequently lost in carcinomas.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; the protein contains ARM repeats and a coiled coil domain.
• Associated with ectodermal dysplasia and other disease variants; alternative splicing generates multiple isoforms with distinct tissue distributions.

Experimental Guidance and Technical Tips

• For Western blotting, consider the predicted molecular weight of 108 kDa and validate using appropriate positive controls (e.g., cell lines expressing δ-catenin).
• As a polyclonal antibody, monitor lot-to-lot consistency and consider titration for your specific experimental conditions.
• The KO validation indicates specificity, so using knockout cell lysates as negative controls is recommended.
• For ELISA, the recombinant immunogen can serve as a positive control; optimize coating concentrations and detection conditions.
• Cross-reactivity with mouse and rat is reported, but confirm reactivity in your model system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to IVD raw materials like the δ-Catenin/p120 Catenin antibody, critical for studying cell adhesion and cancer pathways. Our sourcing network covers validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and rare-target custom development to streamline your project from discovery to clinical application.