Anti-δ-Catenin/p120 Catenin Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - O60716

Core Product Specifications and Parameters

Biological Background: δ-Catenin/p120 Catenin Function and Localization

• Acts as a key regulator of cell-cell adhesion by binding to classical cadherins (C-, E-, N-cadherins) and stabilizing their surface expression, and also facilitates DSG3 localization at intercellular junctions. Related references: PMID:14610055 PMID:18343367 PMID:20371349
• Modulates gene transcription through interactions with transcription factors such as ZBTB33/Kaiso2 and GLIS2, and regulates Rho family GTPase activity to influence cytoskeletal dynamics. Related references: PMID:10207085 PMID:20371349
• Participates in SRC-mediated cell transformation and ligand-induced receptor signaling downstream of EGF, PDGF, CSF-1, and ERBB2 receptors. Related references: PMID:17344476
• Localizes to multiple subcellular compartments including adherens junctions, cytoplasm, nucleus, and cell membrane, reflecting its diverse roles in adhesion and signaling.
• Exhibits tissue-specific isoform expression: vascular endothelium, melanocytes, keratinocytes; the long isoform 1A predominates in melanocytes and melanoma cells, while shorter isoforms, especially 3A, are found in keratinocytes. Isoform 4A is detected in normal cells but often lost in carcinomas. C-terminal exon B inclusion is observed in colon, intestine, and prostate but frequently absent in tumors.
• Undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation, which regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning amino acids 869–968 of human δ-Catenin/p120 Catenin (O60716), lies within a conserved C-terminal region, likely contributing to the reported cross-reactivity with mouse and rat.
• For Western blotting, a primary band at ~108 kDa is expected; additional bands may appear due to isoform diversity or post-translational modifications. Use appropriate loading controls and confirm molecular weight markers.
• In immunohistochemistry (paraffin), antigen retrieval steps (e.g., heat-induced epitope retrieval) may be required. Validate staining in tissue sections known to express the target, such as colon, prostate, or vascular endothelium.
• For immunoprecipitation, the rabbit monoclonal format provides high specificity; however, optimization of antibody concentration and incubation conditions is recommended for each cell lysate.
• ELISA applications should be tested with the specific immunogen peptide or recombinant protein to confirm binding and to establish standard curves.
• Always include relevant negative and positive controls (e.g., knockout cell lines or tissues with known expression) to verify antibody performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, from monoclonal antibodies and antibody pairs to ancillary reagents. This δ-Catenin/p120 Catenin antibody supports cell adhesion and cancer research, and CamelBio can assist with custom antibody development, bulk orders, and rare-target sourcing. Our technical team ensures seamless supply chain integration from concept to clinic.