Anti-δ-Catenin/p120 Catenin Polyclonal Antibody for WB, IHC-P, ELISA - O60716

Core Product Specifications and Parameters

Biological Background: δ-Catenin/p120 Catenin Function and Localization

• δ-Catenin/p120 Catenin (catenin delta-1) is a key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, critical for their surface stability.
  Related references:
  PMID:14610055
  PMID:20371349

• Promotes localization and retention of DSG3 at cell-cell junctions via direct interaction.
  Related references:
  PMID:18343367

• Regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and modulates the activity of Rho family GTPases and downstream cytoskeletal dynamics.
  Related references:
  PMID:10207085
  PMID:20371349

• Implicated in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.
  Related references:
  PMID:17344476

• Localizes to cell junctions (adherens junctions), cytoplasm, nucleus, and cell membrane, reflecting its multifaceted roles.

• Expressed in vascular endothelium; melanocytes and melanoma cells primarily express the long isoform 1A, keratinocytes express shorter isoforms (especially 3A), and the shortest isoform 4A is detected in normal keratinocytes and melanocytes but often lost in tumor-derived cells.

• Belongs to the catenin family and contains Armadillo repeats; undergoes acetylation, phosphorylation, and Ubl conjugation (ISGylation), and is regulated by alternative splicing, contributing to its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 850-968) targets a C-terminal region of human δ-Catenin/p120 Catenin. This region is present in multiple isoforms; however, isoform-specific detection may require further validation.
• For WB, recommended loading controls include beta-actin or GAPDH; optimize antibody dilution based on sample type and detection method.
• IHC-P may require antigen retrieval; test on positive (e.g., human melanoma or keratinocyte) and negative control tissues.
• Cross-reactivity with mouse and rat samples has been reported; validate in your specific experimental model.
• ELISA performance can be influenced by coating conditions; consider sandwich ELISA using this antibody as a capture or detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials and technical support. This anti-δ-Catenin/p120 Catenin polyclonal antibody exemplifies our commitment to delivering validated reagents for investigating cell adhesion, transcriptional regulation, and oncogenic signaling. Beyond single antibodies, we offer matched antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare or complex targets—ensuring you have the critical raw materials needed from concept to clinic.