Anti-δ-Catenin/p120 Catenin Polyclonal Antibody for WB, IHC-P, ELISA - O60716

Core Product Specifications and Parameters

Biological Background: δ-Catenin/p120 Catenin Function and Localization

• δ-Catenin/p120 Catenin (CTNND1) is a member of the catenin family, also known as p120 catenin, cadherin-associated Src substrate (CAS), and p120(CTN).
• It functions as a key regulator of cell-cell adhesion by associating with and stabilizing classical cadherins (C-, E-, and N-cadherins) at the cell surface. Related references: PMID:14610055, PMID:20371349
• δ-Catenin/p120 Catenin promotes the localization and retention of desmoglein-3 (DSG3) at cell-cell junctions, contributing to desmosome integrity. Related references: PMID:18343367
• Beyond adhesion, it regulates gene transcription by interacting with transcription factors such as ZBTB33/Kaiso2 and GLIS2, and modulates Rho family GTPases to influence cytoskeletal dynamics. Related references: PMID:10207085, PMID:20371349
• The protein is implicated in SRC-mediated cell transformation and participates in signaling downstream of receptor tyrosine kinases (EGF, PDGF, CSF-1, ERBB2). Related references: PMID:17344476
• δ-Catenin/p120 Catenin localizes to adherens junctions, the cytoplasm, and the nucleus, with nuclear-cytoplasmic shuttling linked to its roles in transcription and signaling.
• It undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its stability and function.
• Tissue-specific expression includes vascular endothelium and distinct isoform patterns: the long isoform 1A in melanocytes/melanoma, shorter isoforms like 3A in keratinocytes, and isoform 4A in normal keratinocytes and melanocytes, often lost in carcinomas.
• Keywords associated with the protein include Wnt signaling, cell adhesion, and disease variants linked to ectodermal dysplasia.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 573-832 of human δ-Catenin/p120 Catenin (NP_001078938.1). This region may be conserved across species and isoforms; verify sequence alignment for your target of interest.
• For Western blot, the predicted molecular weight is approximately 108 kDa. A band of this size should be observed in lysates from human, mouse, or rat cells expressing the target.
• When performing immunohistochemistry on paraffin-embedded tissues (IHC-P), antigen retrieval conditions and antibody dilution may require optimization. Test on appropriate positive and negative control tissues.
• For ELISA, the antibody can be used as either a capture or detection reagent; assay conditions such as coating concentration and blocking buffer should be empirically determined.
• Cross-reactivity is reported with human, mouse, and rat samples, but reactivity with other species has not been tested; validate if used in other models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This δ-Catenin/p120 Catenin polyclonal antibody supports research in cell adhesion, cancer biology, and signaling pathways. As your sourcing partner, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement to accelerate your assay development and manufacturing processes.