Anti-Cytokeratin AE3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04264/P35908/P12035/P19013/P13647/P02538/P08729

Core Product Specifications and Parameters

Biological Background: Cytokeratin AE3 Function and Localization

• The Cytokeratin AE3 target, primarily keratin 1 (K1), is a type II cytokeratin that forms intermediate filament networks by heterodimerizing with type I keratins, conferring mechanical resilience to suprabasal epidermal keratinocytes (PubMed:1381288). Related references: PMID:1381288
• Keratin 1 modulates intracellular signaling by interacting with integrin β1 (ITB1) and the scaffold protein RACK1, thereby regulating PKC and SRC kinase activities (PubMed:17956333, PubMed:21544310). Related references: PMID:17956333 PMID:21544310
• In complex with the complement component 1 Q subcomponent-binding protein (C1QBP), K1 acts as a high-affinity membrane receptor for kininogen-1 (HMWK), linking coagulation and inflammation pathways (PubMed:21544310). Related references: PMID:21544310
• The protein is predominantly expressed in the cytoplasm and at the cell membrane of terminally differentiating keratinocytes, with highest levels in neonatal foreskin epidermis.
• K1 undergoes several post-translational modifications including phosphorylation, citrullination, and methylation, which influence filament assembly and cellular dynamics.
• Mutations in KRT1 are associated with skin disorders such as epidermolytic hyperkeratosis and palmoplantar keratoderma, highlighting its essential role in epidermal integrity.
• As a member of the large keratin gene family, type II keratins like K1 share structural homology, and this polyclonal antibody may recognize multiple type II cytokeratins (consistent with the listed SWISS accessions).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 181–280 of human keratin 1, a region likely exposed in the filament structure; consider this when designing detection protocols.
• For Western blot (WB), expect bands corresponding to the listed protein weights (51–66 kDa) depending on the specific keratin isoform detected; validate the observed band pattern in your sample system.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate buffer (pH 6.0) and careful titration may improve signal in formalin-fixed paraffin-embedded tissues.
• For ELISA, both direct and indirect formats can be explored, considering the polyclonal nature of the antibody for high sensitivity.
• Given potential cross-reactivity with other type II keratins, inclusion of appropriate controls (e.g., knockout cell lines or specific blocking peptides) is recommended to confirm target identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, bulk ancillary reagents, and custom sourcing solutions for research and diagnostic development. This anti-Cytokeratin AE3 antibody supports studies in epithelial biology, dermatopathology, and keratin-related disorders. Contact us to access validated antibody pairs, optimized monoclonals/polyclonals, and rare-target sourcing tailored to your requirements.