Anti-Cytokeratin 9 (KRT9) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P35527

Core Product Specifications and Parameters

Biological Background: Cytokeratin 9 (KRT9) Function and Localization

• Cytokeratin 9 (KRT9), also known as Keratin-9 (K9) or CK-9, is a type I cytoskeletal keratin encoded by the KRT9 gene in humans.
• It serves as a structural component of intermediate filaments specifically in suprabasal keratinocytes of the palmoplantar epidermis, assembling with type II keratins to form robust keratin networks.
• These keratin intermediate filament networks are essential for maintaining the mechanical integrity and resilience of the palm and sole skin, withstanding significant pressure and friction.
• Expression of KRT9 is tightly restricted to the terminally differentiated epidermis of palms and soles, highlighting its specialized role in these high-stress body sites.
• Mutations in KRT9 are associated with epidermolytic palmoplantar keratoderma (EPPK), an autosomal dominant skin disorder characterized by diffuse thickening of palm and sole skin.
• The protein structure features a central coiled-coil rod domain typical of intermediate filament proteins, facilitating dimerization and filament assembly.
• Post-translational regulation may include phosphorylation events, as indicated by proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 150–450 of human KRT9, an internal region. This design may allow detection of total KRT9 protein in various sample types.
• For Western blot (WB), use denaturing and reducing conditions, and consider including a loading control such as GAPDH or β-actin. The predicted molecular weight is approximately 62 kDa; validate band identity using appropriate positive (e.g., palm skin lysate) and negative controls.
• For immunofluorescence (IF-P), fixation and permeabilization methods should be optimized. Since KRT9 is a cytoplasmic intermediate filament, permeabilization is essential. Include a nuclear counterstain like DAPI to visualize cellular localization.
• For ELISA applications, titrate the antibody using recombinant KRT9 or cell lysates to determine optimal working dilutions. Pairing with a matched detection antibody may improve sensitivity.
• Cross-reactivity is reported for human, mouse, and rat; however, verify specificity in the relevant species and tissue context, as expression is limited to palm and sole epidermis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers, laboratories, and research institutes, offering a broad portfolio of validated antibodies and ancillary reagents. For studies focused on cytokeratin biology, palmoplantar skin differentiation, or keratinization disorders, we supply premium raw materials including optimized polyclonal and monoclonal antibodies, recombinant proteins, and bulk ancillary reagents. Additionally, CamelBio facilitates the sourcing of rare-target antibodies and supports concept-to-clinic development with technical consultation and reliable supply chains.