Anti-Cytokeratin 9 (KRT9) Rabbit Monoclonal Antibody for WB, ELISA - P35527

Core Product Specifications and Parameters

Biological Background: Cytokeratin 9 (KRT9) Function and Localization

• Cytokeratin 9 is a type I intermediate filament protein (keratin) primarily expressed in the suprabasal layers of the palmoplantar epidermis.
• It serves as a structural component, forming heteropolymers with type II keratins to assemble the keratin intermediate filament network, thereby maintaining the mechanical resilience of palm and sole skin.
• The protein is characterized by a coiled-coil domain and undergoes phosphorylation, which may regulate filament dynamics.
• Mutations in KRT9 are associated with epidermolytic palmoplantar keratoderma, an autosomal dominant skin disorder.
• Tissue expression is restricted to the terminally differentiated epidermis of palms and soles, marking it as a specific marker for these sites.

Experimental Guidance and Technical Tips

• For Western blot, a band around 62 kDa is expected; use denaturing and reducing conditions, and include appropriate positive controls such as lysates from palmoplantar skin or cell lines expressing KRT9.
• For ELISA, consider testing the antibody in both direct and sandwich formats; pair with a suitable type II keratin antibody for specific detection.
• Due to cross-reactivity with mouse and rat, verify reactivity in the relevant species samples; sequence alignment of the immunogen region (aa 254-412) shows high conservation among these species, but empirical validation is recommended.
• The antibody may detect phosphorylated and unphosphorylated forms; treat samples with phosphatase inhibitors if needed.

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