Anti-Cytokeratin 8 (KRT8) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8 (KRT8) Function and Localization

• Serves as a structural component of keratin intermediate filaments in simple epithelial cells, partnering with KRT18 to form a network that provides mechanical stability and resilience (PubMed:26823019). Related references: PMID:26823019
• Helps maintain epithelial integrity and protects cells from non-mechanical stress, including apoptosis (By similarity).
• Required for ARHGEF40-mediated actin stress fiber formation and reinforcement in response to tensional forces (PubMed:26823019). Related references: PMID:26823019
• In striated muscle, interacts with KRT19 to link the contractile apparatus to dystrophin at costameres (PubMed:16000376). Related references: PMID:16000376
• Localizes to the cytoplasm, nucleoplasm, nucleus matrix, and cytoskeleton.
• Detectable in muscle fibers accumulating in costameres of myoplasm, and expressed in gingival mucosa and hard palate.
• Post-translational modifications include acetylation, phosphorylation, glycosylation, methylation, and ubiquitination; classified under keratins, intermediate filaments, and disease-associated variants.

Experimental Guidance and Technical Tips

• For Western blot (WB), the immunogen spans the full-length KRT8 protein; the predicted molecular weight is ~53.7 kDa, but post-translational modifications may result in bands at 54-56 kDa. Optimize loading controls and antibody dilutions empirically.
• In immunohistochemistry (IHC-P), consider heat-induced antigen retrieval methods and appropriate tissue fixation protocols to unmask epitopes; validate with known positive and negative tissue controls.
• For immunofluorescence (IF/ICC), permeabilization steps may be required for intracellular target detection; test different blocking conditions to minimize background.
• The antibody shows cross-reactivity with mouse KRT8, but sequence alignment near the immunogen region may help predict reactivity in other species; consider sequence homology analysis.
• As a polyclonal antibody, lot-to-lot consistency may vary; always titrate the antibody for each new application or experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-Cytokeratin 8 antibody is part of our extensive catalog for intermediate filament and epithelial cell biology research. We offer validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and customized sourcing solutions for rare targets to accelerate your assay development and diagnostic workflows.