Anti-Cytokeratin 8 (CK8) Monoclonal Antibody for WB and ELISA - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8 Function and Localization

• Keratin 8 (KRT8) is a type II intermediate filament protein, also known as cytokeratin-8 (CK-8), K8, or CYK8, and is encoded by the KRT8 gene in humans.
• Together with its obligate type I partner KRT18, KRT8 forms heteropolymeric keratin intermediate filaments that provide mechanical stability and resilience to simple epithelial cells. Related references: PMID:26823019
• In addition to mechanical support, KRT8 contributes to the protection of epithelial cells from non-mechanical stress, such as apoptosis.
• KRT8 is also required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber reinforcement, linking intermediate filaments to the actin cytoskeleton. Related references: PMID:26823019
• In striated muscle, KRT8 may cooperate with KRT19 to link the contractile apparatus to dystrophin at costameres, contributing to muscle cell integrity. Related references: PMID:16000376
• Subcellularly, KRT8 is found in the cytoplasm, nucleus, nucleoplasm, and nuclear matrix, reflecting its roles beyond mere cytoskeletal architecture.
• Tissue expression includes simple epithelia, with notable accumulation in muscle costameres, gingival mucosa, and hard palate of the oral cavity.
• Post-translational modifications of KRT8 include phosphorylation, acetylation, methylation, and glycosylation, which regulate filament dynamics and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider that the antibody likely recognizes linear epitopes, making it well-suited for Western blot under denaturing conditions.
• For ELISA, evaluate both direct and indirect formats, and validate working concentrations in your specific assay system.
• Given cross-reactivity with mouse and rat, confirm signal specificity in these species using appropriate negative controls.
• Protein weight appears as a doublet around 54–56 kDa; ensure adequate gel resolution and consider loading controls to distinguish specific bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cytokeratin 8 research, we supply a range of anti-keratin antibodies suitable for diagnostic assay development, from concept to clinic. Whatever your rare target or large-volume need, CamelBio bridges the gap between discovery and commercial production.