Anti-Cytokeratin 7 (KRT7) Monoclonal Antibody for WB, IHC-P, IF, ELISA - P08729

Core Product Specifications and Parameters

Biological Background: Cytokeratin 7 Function and Localization

• Keratin 7 (KRT7) is a type II cytoskeletal keratin, a member of the intermediate filament family, encoded by the KRT7 gene (also known as SCL). Alternative names include CK-7, K7, sarcolectin, and type-II keratin Kb7.
• KRT7 blocks interferon-dependent interphase and stimulates DNA synthesis, thereby influencing cell proliferation.
• It is involved in the translational regulation of human papillomavirus type 16 (HPV16) E7 mRNA, implicating a role in host-virus interactions.
• Subcellular localization is primarily cytoplasmic, consistent with its function as an intermediate filament protein.
• Tissue expression is observed in cultured epidermal, bronchial, and mesothelial cells, but absent in colon, ectocervix, and liver; it is expressed in glandular cells of the gastroesophageal junction but not in the esophagus.
• The protein undergoes several post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitin conjugation (Ubl), as well as forming isopeptide bonds. It is also a glycoprotein.
• KRT7 has a molecular weight of approximately 51 kDa (calculated ~51.4 kDa), consistent with the supplier-provided weight.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated against a synthetic peptide corresponding to amino acids 1–100 of human KRT7 (P08729). The immunogen lies in the N-terminal region, which may be well-exposed in the native protein.
• For Western blotting (WB), denaturing and reducing conditions are recommended; a band at ~51 kDa is expected. Validate the antibody in a relevant cell or tissue lysate expressing KRT7.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), consider antigen retrieval methods (e.g., heat-induced epitope retrieval) and appropriate fixation to preserve epitope integrity. Validate in positive control tissues such as glandular epithelium or bronchial cells.
• The antibody shows cross-reactivity with mouse and rat KRT7 orthologs, as per supplier data, but cross-reactivity should be confirmed experimentally in the relevant species and application.
• As with any monoclonal antibody, lot-to-lot consistency should be monitored, and optimal working dilutions determined empirically for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a wide range of IVD raw materials, backed by technical services and consulting from concept to clinic. This anti-KRT7 rabbit monoclonal antibody is part of our portfolio supporting epithelial cell biology, cancer biomarker research, and pathology applications. Beyond validated monoclonal and polyclonal antibodies like this one, we also supply optimized antibody pairs, bulk ancillary reagents, and can assist in sourcing raw materials for rare or difficult targets.