Anti-Cytokeratin 7 (KRT7) Monoclonal Antibody for WB, IF, IHC, ELISA - P08729

Core Product Specifications and Parameters

Biological Background: Cytokeratin 7 (KRT7) Function and Localization

• KRT7 encodes keratin, type II cytoskeletal 7, a member of the keratin family of intermediate filament proteins. Alternative names include cytokeratin-7 (CK-7), keratin-7 (K7), sarcolectin, and type-II keratin Kb7.
• It plays a role in blocking interferon-dependent interphase and stimulating DNA synthesis, and is involved in translational regulation of HPV16 E7 mRNA, indicating a host-virus interaction.
• The protein localizes to the cytoplasm, as part of the intermediate filament network, contributing to cellular structural integrity.
• Tissue specificity: expressed in cultured epidermal, bronchial, and mesothelial cells, but absent in colon, ectocervix, and liver. Observed in glandular cells at the gastroesophageal junction but not in the esophagus, making it a useful marker for certain epithelial lineages.
• Post-translational modifications include acetylation, glycoprotein, isopeptide bond, methylation, phosphorylation, and Ubl conjugation, which may regulate its function.
• The protein has a coiled-coil domain and is involved in intermediate filament assembly. Its molecular weight is approximately 51.4 kDa (UniProt) / 51 kDa (observed).
• Additional keywords: 3D-structure, direct protein sequencing, proteomics identification, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 291-463) of human KRT7, which is relatively conserved; cross-reactivity is expected with mouse and rat. Consider validating specificity in the relevant model system prior to use.
• For Western blotting, the predicted molecular weight is 51 kDa; verify band location using appropriate molecular weight markers and loading controls.
• In immunohistochemistry (IHC) and immunofluorescence (IF), antigen retrieval may be necessary depending on tissue preparation; optimize fixation and permeabilization conditions.
• ELISA suitability suggests potential for quantitative detection assays. Titration may be required for optimal signal-to-noise ratio.
• Store as recommended (ice bag shipping indicates cold-chain requirement); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-Cytokeratin 7 monoclonal antibody supports epithelial lineage research and assay development; beyond this product, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents for streamlined kit manufacturing. Our sourcing capabilities encompass rare targets, ensuring you find the critical raw materials needed for your diagnostic projects.