Anti-Cytokeratin 6 (KRT6) Monoclonal Antibody for WB, IHC-P, ELISA - P48668

Core Product Specifications and Parameters

Biological Background: Cytokeratin 6 Function and Localization

• Keratin 6C (KRT6C) is a type II cytokeratin, also designated CK-6C, CK-6E, or K6C, encoded by the KRT6C gene (alternative name KRT6E).
• It serves as a structural component of intermediate filaments in epithelial keratinocytes, forming heteropolymers with type I keratins such as KRT16 and KRT17 to establish robust keratin networks.
• These keratin intermediate filaments provide mechanical resilience and integrity to stratified epithelia, particularly in the palmoplantar epidermis.
• KRT6C is constitutively expressed in specific epithelial tissues including oral mucosa, esophagus, tongue papillae, and the outer root sheath of hair follicles.
• Post-translational modifications include acetylation and phosphorylation, which may regulate filament assembly, dynamics, and interaction with associated proteins.
• Mutations in KRT6C are associated with palmoplantar keratoderma, underscoring its critical role in maintaining epidermal barrier function.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of KRT6C is approximately 60 kDa; consider using appropriate protein ladder and validate band specificity in positive control lysates (e.g., cultured keratinocytes or epithelial tissue extracts).
• For immunohistochemistry (paraffin), antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer pH 6.0) should be optimized; include negative and positive tissue controls (e.g., skin sections).
• For ELISA, pair this antibody with a suitable detection antibody; the peptide immunogen may be used as a positive control.
• Note: The antibody detects only human KRT6; cross-reactivity with other species or other keratin family members should be experimentally verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material procurement, offering an integrated portfolio from validated antibody pairs to bulk ancillary reagents. This anti-Cytokeratin 6 (KRT6) monoclonal antibody facilitates research into epithelial biology, keratinocyte function, and dermatological conditions. For customized sourcing of rare targets or optimized reagent combinations, our technical team is ready to support your development pipeline.